In October 1890, the younger generation of Swedish immigrants, whose parents had been persuaded to settle here by Henry Sanford, organized a Swedish Presbyterian congregation along American lines. In 1891 the congregation was enrolled in the South Florida Presbytery. 

Upsala Presbyterian Church, primarily located in the New Upsala, Florida area, was founded in 1891 by Swedish immigrants who converted to Presbyterianism, with the name "Upsala" likely referencing the city of Uppsala in Sweden; the younger generation of Swedish settlers in the community chose to establish a Presbyterian church separate from the existing Lutheran church, building their own church on Upsala Road.  

• Swedish origins:
  The church was founded by Swedish immigrants who settled in the New Upsala area, a community with a strong Scandinavian heritage. 

• Name origin:
  "Upsala" is derived from Uppsala, a prominent city in Sweden. 

• Conversion to Presbyterianism:
  While initially attending a Scandinavian Society Church, many Swedish settlers converted to Presbyterianism under the influence of missionaries, leading to the establishment of Upsala Presbyterian Church.